Immune response variations to Salmonella enterica serovar Typhi recombinant porin proteins in mice
پنجشنبه , 24 مرداد 1392 ساعت 03:35 دسته بندی : علم زندگی ,دانلود مقاله :
Immune response variations to Salmonella enterica serovar Typhirecombinant porin proteins in mice 2013
نویسندگان :
Hoda Toobak , Iraj Rasooli , Daryush Talei , Abolfazl Jahangiri , Parviz Owlia ,Shakiba Darvish Alipour Astaneh
چکیده :
Objectives: Typhoid fever is caused by Salmonella enterica serovar Typhi. OmpC, OmpF and OmpA, the
three major outer membrane proteins (OMPs), could serve as vaccine candidates.
Methods: The porins antigenicity was predicted in silico. The OMP genes were amplified, cloned and
expressed. Sero-reactivities of the recombinant proteins purified by denaturing method were assayed by
ELISA. BALB/c mice were immunized with the recombinant porins followed by bacterial challenge.
Results: Bacterial challenge of the animal model brought about antibody triggering efficacy of the antigen
in OmpF > OmpC > OmpA order. Experimental findings validated the in silico results. None of the antigens
had synergic or antagonistic effects on each other from immune system induction points of view.
Despite their high immunogenicity, none of the antigens was protective. However, administration of two
or three antigens simultaneously resulted in retardation of lethal effect. Porins, in addition to their
specific functions, share common functions. Hence, they can compensate for each other’s functions.
Conclusions: The produced antibodies could not eliminate the pathogenicity by blockade of one or some
of the antigens. Porin antigens are not suitable vaccine candidates alone or in denatured forms. Native
forms of the antigens maybe studied for protective immunogenicity.
?> OmpC OmpF OmpA Salmonella enterica ssp Typhi Immunity , دانلود مقاله بیوشیمیمطالب مرتبط :
Long-term treatment with deferiprone enhances left ventricular ejection function when compared to deferoxamine in patients with thalassemia major
Signal-dependent Elk-1 target genes involved in transcript processing and cell migration
Subtype-specific roles of phospholipase C-b via differential interactions with PDZ domain proteins
Physiology and pathology of nuclear phospholipase C b1
Social context influences the initiation and threshold of thermoregulatory behaviour in honeybees
Divergent selection on bill morphology contributes to nonrandom mating between swamp sparrow subspecies
Suppressive effects of seed-applied fungicides on arbuscularmycorrhizal fungi (AMF) differ with fungicide mode ofaction and AMF species
Changes of microbial properties in (near-) rhizosphere soils afterphytoextraction by Sedum alfredii H: A rhizobox approach withan artificial Cd-contaminated soil
Impacts of chloride de-icing salt on bulk soils, fungi, and bacterialpopulations surrounding the plant rhizosphere
Analysis of the non-thermophilic Crenarchaeota phylogeny in the swamp soil of Zoige plateau wetland
A study on early tolerance of Mactra chinensis philippi to salinity
Carbon footprints of rice production in five typical rice districts in China
Rapid clearance of intranasally administered DNA from rat tissues
Yellow fever vaccine: Comparison of the neurovirulence of new 17D-204 Stamaril
Statistical challenges in the analysis of dynamic traits: Implications for pharmacogenomic clinics
Immune response variations to Salmonella enterica serovar Typhi recombinant porin proteins in mice
The combined behavioural tendencies of predator and prey mediate the outcome of their interaction
Development of aggressive phenotypes in zebrafish: interactions of age, experience and social status
A method for automated individual, species and call type recognition in free-ranging animals
Do ship rats display predatory behaviour towards house mice?
Life history variation in male mate choice in Drosophila melanogaster
Multitasking males and multiplicative females: dynamic signalling and receiver preferences in Cope’s grey treefrog
How do environmental factors and different fertilizer strategies affectsoil CO2emission and carbon sequestration in the upland soils ofsouthern China?
Effect of elevated atmospheric CO2and temperature on diseaseseverity of Fusarium oxysporum f.sp. lactucae on lettuce plants
Trichoderma harzianum T-E5 significantly affects cucumber rootexudates and fungal community in the cucumber rhizosphere
A review on estimation of stochastic differential equations for pharmacokinetic/ pharmacodynamic models
Stochastic modeling of systems mapping in pharmacogenomics
Dedicated mothers: predation risk and physical burden do not alter thermoregulatory behaviour of pregnant vipers
Pseudoreplication: a widespread problem in primate communication research
Evolution of individual variation in behaviour and behavioural plasticity under scramble competition
Phage display antibodies for diagnostic applications
Personalized medicine: A paradigm shift in healthcare
Genetic variations within the PSORS1 region affect Kawasaki disease development and coronary artery aneurysm formation
Appearance of acanthosis nigricans may precede obesity: An involvement of the insulin/IGF receptor signaling pathway
Biotechnology advances: A perspective on the diagnosis and research of Rabies Virus
Increased incidence of Parkinsonism among Chinese with b-glucosidase mutation in central Taiwan
Association analysis between Tourette’s syndrome and two dopamine genes (DAT1, DBH) in Taiwanese children
Selfish behaviour as an antipredator response in schooling fish?
Corncrake males learn new signal meanings during aggressive interactions
Inversion of lefteright asymmetry alters performance of Xenopus tadpoles in nonlateralized cognitive tasks
Trichoderma harzianum T-E5 significantly affects cucumber rootexudates and fungal community in the cucumber rhizosphere
The rhizosphere soil of diseased tomato plants as a source for novelmicroorganisms to control bacterial wilt 2013